منابع مشابه
The separation of rat liver serine dehydratase and cystathionine synthase.
A compound derived from pyruvic acid and homocysteine has been isolated from reaction mixtures containing serinel*C, homocysteine, and serine dehydratase preparations. The formation of this product interferes with commonly used assays of serine dehydratase, which are based on the serine, pyruvic acid, or homocysteine content of incubation mixtures. Improved assay procedures have permitted the i...
متن کاملCrystal structure of the pyridoxal-5'-phosphate-dependent serine dehydratase from human liver.
L-serine dehydratase (SDH), a member of the beta-family of pyridoxal phosphate-dependent (PLP) enzymes, catalyzes the deamination of L-serine and L-threonine to yield pyruvate or 2-oxobutyrate. The crystal structure of L-serine dehydratase from human liver (hSDH) has been solved at 2.5 A-resolution by molecular replacement. The structure is a homodimer and reveals a fold typical for beta-family...
متن کاملCrystallization and preliminary crystallographic analysis of human serine dehydratase.
L-Serine dehydratase (SDH) catalyzes the pyridoxal phosphate (PLP) dependent deamination of L-serine to yield pyruvate. Recombinant human serine dehydratase was crystallized by the hanging-drop vapour-diffusion method. Crystals were grown at 291 K using (NH4)(2)SO4 as precipitant. Diffraction data were obtained to a resolution of 2.5 A from a single frozen crystal using Cu Kalpha radiation. The...
متن کاملLocalization of Polysome-bound Albumin and Serine Dehydratase in Rat Liver Cell Fractions
The polysomes involved in albumin and serine dehydratase synthesis were identified and localized by the binding to rat liver polysomes of anti-rat serum albumin and anti-serine dehydratase [(125)I]Fab dimer and monomer. Techniques were developed for the isolation of undegraded free and membrane-bound polysomes and for the preparation of [(125)I]Fab monomers and dimers from the IgG obtained from...
متن کاملRat serine dehydratase gene codes for two species of mRNA of which only one is translated into serine dehydratase.
With the previously obtained rat liver serine dehydratase cDNA (SDH2; Ogawa, H., Miller, D.A., Dunn, T., Su, Y., Burcham, J. M., Peraino, C., Fujioka M., Babcock, K., and Pitot, H. C. (1988) Proc. Natl. Acad. Sci. U.S. A. 85, 5809-5813) as a probe, we isolated a different species of cDNA (SDH3) from the same cDNA library from which SDH2 was obtained. Nucleotide sequence analysis has indicated t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)71550-0